Project no. 331
‘DeTectUs’: Rapid Detection of the Level of
Vancomycin Antibiotic in Blood Plasma
A.P. Rasmussen3, B. Gezer4, C. Hermansen1, K. Steenberg2, K. Holst4, M.
Lind3, M. G. Petersen2, M. El Lakany6, N. Støvrin2, S. G. L. Pedersen2, Z.
1DTU Pharmaceutical Engineering, Technical University of Denmark
2DTU Micro- and Nanotechnology, Technical University of Denmark
3DTU Management Engineering, Technical University of Denmark
4DTU Electrical Engineering, Technical University of Denmark
5DTU Bioengineering, Technical University of Denmark
6DTU Bioinformatics, Technical University of Denmark
A point of care device for the detection of vancomycin levels in blood plasma in a fast, easily
and accurately manner in order to accommodate the busy schedules of doctors and nurses
at Danish hospitals.
Vancomycin is indicated for the treatment of serious, life-threatening infections by
Gram-positive bacteria unresponsive to other antibiotics. It is therefore used as an last resort
option. Subinhibitory levels is indicated to lead to the development and transfer of resistance
genes to non harmful bacteria leading to volatile multi-resistant organisms.
Presently, the doctors can order a laboratory technician to perform the vancomycin test but
each department only have specific time slots where tests can be taken. du to this and the
processing time for a test, the the doctor who ordered the test will not get the result during
his/her shift. Thus, the next doctor in shift will have to use valuable time finding out what to
We identified viable peptide and molecular imprinted polymers (MiPs) biomarker candidates
responsive to vancomycin. We employ these to detect vancomycin by functionalizing these
to a surface to a transducing surface: Gold electrodes and silicon nanowires (SiNW). The
binding of the vancomycin molecules affects the current which yield an observable
change in the signal – the conductance sensitivity. These binding events and its
corresponding change in flow of current is then translated to an electrical readout in nano
and micro Ampere We further incorporate the ability for data transmission to the
databases employed at the respective hospitals for ease of access for doctors.
Ready to use point of care devices for rapid detection of the antibiotic vancomycin at
the bedside of the patient, will enable for better treatment strategies in hospitals. Thus
alleviating the issue of logistics and not having to wait for results from a central laboratory. In
addition this will save valuable resources at the hospital by having the option of sending
patients home, if they are fit enough, in order to return for the next administering of antibiotic
the next day.